Focusing on the issue of target motion feature extraction of video sequences in complex scene, and referring to the motion perception of biological vision system to the moving video targets, the traditional primary Visual cortex (V1) cell model of visual cortex was improved and a novel method of random-dot motion feature extraction based on the mechanism of biological visual cortex was proposed. Firstly, the spatial-temporal filter and half-squaring operation combined with normalization were adopted to simulate the linearity and nonlinearity of neuron's receptive field. Then, a universal V1 cell model was obtained by adding a directional selectivity adjustable parameter to the output weight, which solved the problem of the single direction selectivity and the disability to respond correctly to multi-direction motion in the traditional model. The simulation results show that the analog outputs of proposed model are almost consistent with the experimental data of biology, which indicates that the proposed model can simulate the V1 neurons of different direction selectivity and extract motion features well from random-dot video sequences with complex motion morphs. The proposed method can provide new idea for processing feature information of optical flow, extract motion feature of video sequence and track its object effectively.